Thermo Fisher Scientific Erythroid Cells Monoclonal Antibody (HIS49), FITC, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

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Product Specifications

Species Reactivity

Rat

Published species

Rat

Host/Isotype

Mouse / IgM, kappa

Recommended Isotype Control

Mouse IgM Isotype Control (11E10), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HIS49

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572536

Product Specific Information

Description: The monoclonal antibody HIS49 reacts with an unknown antigen expressed by rat erythroid cells and their progenitors. HIS49 does not react with lymphocytes, monocytes or granulocytes.

Applications Reported: This HIS49 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIS49 antibody has been tested by flow cytometric analysis of rat bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.