Thermo Fisher Scientific p27 Kip1 Polyclonal Antibody
p27 Kip1 단백질을 검출하는 Rabbit Polyclonal Antibody로, WB 및 ICC/IF에 적합함. 인간, 생쥐, 랫트에서 반응하며 고순도 Affinity chromatography로 정제됨. PBS/glycerol 저장 버퍼로 안정적이며 연구용으로 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CDKN1B (Accession P46527), corresponding to amino acid residues S161–T198 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2900337 |
Product Specific Information
Antibody detects endogenous levels of total p27 Kip1.
Target Information
p27 Kip1 functions as a negative regulator of G1 phase progression and may mediate TGFβ-induced G1 arrest. It is a key regulator of cell cycle progression, inhibiting cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 also interacts with cyclin D-CDK4 complexes, influencing their assembly and activation depending on its phosphorylation state. The protein (~27 kDa) undergoes CDK-dependent phosphorylation and ubiquitination by SCF complexes, enabling the transition from quiescence to proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: PA5-115702_p27Kip1_P46527-1_Rabbit.svg, PA5-115702_p27Kip1_P46527-1_Rabbit_PDP.jpeg)
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