Thermo Fisher Scientific IL-3 Monoclonal Antibody (4806)
인간 IL-3에 특이적인 Mouse IgG1 단클론 항체로, Western blot, ICC/IF, Neutralization 등 다양한 분석에 적합. E. coli 유래 재조합 IL-3 항원으로 제작되었으며, 교차 반응 없음. 단백질 A/G 정제, 동결건조 형태로 제공되며 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 8–25 µg/mL |
| Neutralization (Neu) | 0.03–0.08 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4806 |
| Immunogen | E. coli-derived recombinant human IL-3 (Ala20–Phe152) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609600 |
Product Specific Information
- In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL-3 is observed.
- This antibody does not neutralize the biological activity of recombinant mouse IL-3.
- Reconstitute at 0.5 mg/mL in sterile PBS.
- Endotoxin level is <0.10 EU per 1 µg of the antibody (LAL method).
Target Information
Interleukin 3 (IL-3), also known as Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells, and eosinophils.
IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell, and monocytic lineages.
Most of these functions are enhanced or dependent on co-stimulation with other cytokines.
Human and mouse IL-3 share only 29% sequence identity at the amino acid level, and bioactivity is highly species-specific — human IL-3 has no activity on mouse cells.
IL-3 supports proliferation, differentiation, and apoptosis of hematopoietic cells, and also possesses neurotrophic activity possibly associated with neurologic disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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