Thermo Fisher Scientific BDNF Polyclonal Antibody
BDNF 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal 항체. Western blot, IHC, ICC 등 다양한 응용 가능. 인간, 마우스, 랫 등 반응성 확인됨. 항원 친화 크로마토그래피로 정제된 고품질 시약.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 4 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human BDNF |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.52 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792869 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Dog (100%), Cat (100%), Pig (100%), Chimpanzee (100%), Bovine (100%).
- Positive Control: mouse brain, rat brain, BDNF-transfected 293T.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and secreted in synaptic vesicles along with the mature form.
BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor p75-NTR has also been demonstrated.
BDNF promotes neuronal survival and differentiation and plays a critical role in memory formation and synaptic regulation. Expression is reduced in Alzheimer’s and Huntington disease patients and may also be involved in stress response and mood disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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