Thermo Fisher Scientific Phospho-AKT1 (Ser473) Polyclonal Antibody
Phospho-AKT1 (Ser473) 폴리클로날 항체로 인간, 마우스, 랫 등 다양한 종에서 반응. Western blot에 적합하며 세포 생존 및 신호전달 연구에 사용. 항원 친화 크로마토그래피로 정제된 고품질 항체로 안정적인 액상 형태로 제공.
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Applications
- Western Blot (WB): Tested dilution 1:500–1:3,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human AKT (phospho Ser473/474/472). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.31 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792868 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Cat (100%), Pig (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%).
- Positive Control: MCF-7, PC-12, Rat2, NIH-3T3 (5 ng/ml PDGF treatment for 15 min).
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1, and other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors and caspase-9. The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: PA5-85729_AKT1_P31749-1_Rabbit.svg, PA5-85729_AKT1_P31749-1_Rabbit_PDP.jpeg)
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