Thermo Fisher Scientific Human DFFB Synthetic Peptide

Applications

Control (Ctrl)

• Assay-dependent

Blocking Assay (BLOCK)

• Assay-dependent

Product Specifications

Product Specific Information

This peptide corresponds to 16 amino acids at the amino terminus of human DFF40.
PEP-0053 can be used as a blocking peptide with polyclonal antibody PA5-19928.

Target Information

Caspase-activated DNase (CAD) cleaves chromosomal DNA during apoptosis. Apoptosis, a cell death process removing toxic and/or useless cells during mammalian development, can be induced by various stimuli including death factors of the tumor necrosis factor family, UV irradiation, anticancer drugs, and survival factor deprivation.
The apoptotic process is accompanied by shrinkage and fragmentation of cells and nuclei and degradation of chromosomal DNA into nucleosomal units. CAD, which can be activated by caspases, exists as a complex with its inhibitor ICAD (inhibitor of CAD). ICAD assists in proper folding of CAD and remains complexed with CAD to inhibit its DNase activity.
During apoptosis, activated caspases cleave ICAD, releasing CAD, which then enters the nucleus to cleave chromosomal DNA. Both internal and external signals can activate caspases, regulated by the balance between pro- and anti-apoptotic proteins of the Bcl-2 family, heat shock proteins, and inhibitors of apoptosis proteins (IAPs). Fourteen members of the caspase family have been identified, seven of which mediate apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.