Thermo Fisher Scientific Cytokeratin 5 Monoclonal Antibody (2C2)
Cytokeratin 5 단백질 검출용 Mouse monoclonal antibody (Clone 2C2). Human, Mouse, 비인간 영장류 시료에 반응. WB, IHC, ICC, Flow, ELISA 등 다양한 응용 가능. 단백질 G 정제, 액상 형태, 장기보관 시 -20°C 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 2 publications |
| Immunohistochemistry (IHC) | - | View 7 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | View 1 publication |
| Flow Cytometry (Flow) | 1:200–1:400 | View 1 publication |
| ELISA | 1:10,000 | View publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate |
| Published Species | Cynomolgus monkey, Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2C2 |
| Immunogen | Purified recombinant fragment of human CK5 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538529 |
Product Specific Information
MA5-17057 targets CK5 in indirect ELISA, FACS, ICC, IHC, IF, and WB applications, showing reactivity with Human, Mouse, and Non-human primate samples.
The immunogen is a purified recombinant fragment of human CK5 expressed in E. coli.
MA5-17057 detects CK5 with a predicted molecular weight of approximately 62.3 kDa.
Target Information
Cytokeratin pan is part of a subfamily of intermediate filament proteins characterized by biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides.
Cytokeratins range in molecular weight between 40–68 kDa and have an isoelectric pH between 4.9–7.8.
Human cytokeratins are numbered 1–20, and their expression patterns vary by epithelium type and maturation stage.
These patterns are valuable for distinguishing epithelial malignancies and are used in immunohistochemistry, cytopathology, and flow cytometric assays.
Keratins serve as markers in cancer research and tumor diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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