Thermo Fisher Scientific MLCK Polyclonal Antibody
Rabbit polyclonal antibody against human MLCK; validated for WB and ICC/IF; affinity-purified with >95% purity; supplied as liquid in PBS with glycerol; ideal for studying muscle contraction and MLCK signaling pathways.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant full length Human MLCK |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2720709 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. Purity is >95% (by SDS-PAGE).
Target Information
MLCK (MLCK2), also known as MYLK3, is a myosin regulatory light chain kinase facilitating muscle contraction. It phosphorylates myosin light chains, enabling actin-myosin interaction. MLCK is crucial for vascular permeability regulation, tissue edema formation, and neural signaling processes. It participates in fibroblast apoptosis signaling and is expressed in various adult and fetal tissues. The protein contains one fibronectin type III domain and nine immunoglobulin-like C2-type domains.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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