Thermo Fisher Scientific CHOP Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:400

-

Immunocytochemistry (ICC/IF)

5-10 µg/mL

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide, conjugated to KLH, corresponding to residues K-E(153) A T R R A L I D R M V N L H Q A(169) of human DDIT3, with an N-terminal added if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CHOP,uniProtId:P35638-1,ncbiNodeId:9606,antigenRange:153-169,antigenLength:169,antigenImageFileName:PA1-24697_CHOP_P35638-1_Rabbit.svg,antigenImageFileNamePDP:PA1-24697_CHOP_P35638-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.3 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2230268

Product Specific Information

Recommended positive controls: HeLa.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

GADD153 is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP GADD153 dimer gains binding activity to other non classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of GADD153 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.