Thermo Fisher Scientific RXRB Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

-

ChIP assay (ChIP)

-

View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Bovine, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues Q(190) K S D Q G V E G P G A T(202) of mouse RXR beta. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RXRB,uniProtId:P28704-1,ncbiNodeId:10090,antigenRange:190-202,antigenLength:520,antigenImageFileName:PA1-815_RXRB_P28704-1_Rabbit.svg,antigenImageFileNamePDP:PA1-815_RXRB_P28704-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2182763

Product Specific Information

PA1-815 detects retinoid X receptor (RXR) beta from human and mouse cells as well as transfected human and mouse RXR beta.

PA1-815 has been successfully used in Western blot, immunoprecipitation and gel shift procedures. By Western blot, this antibody detects an ~50 kDa protein representing RXR beta from COS cells transfected with the human gene. Gel shift assays reveal supershifting of vitamin D receptor (VDR)/RXR beta/vitamin D receptor response element (VDRE) complexes when PA1-815 is preincubated with RXR beta prior to addition of VDR.

The PA1-815 immunogen is a synthetic peptide corresponding to residues Q(190) K S D Q G V E G P G A T(202) of mouse RXR beta.

Recombinant human VDR (Cat. # RP-710) and human RXR beta (catalog # RP-812) proteins can be purchased separately for control experiments in Western blot, immunoprecipitation and gel shift procedures.

Target Information

This gene encodes a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid (RA). This receptor forms homodimers with the retinoic acid, thyroid hormone, and vitamin D receptors, increasing both DNA binding and transcriptional function on their respective response elements. The gene lies within the major histocompatibility complex (MHC) class II region on chromosome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.