Thermo Fisher Scientific ATG16L1 Recombinant Rabbit Monoclonal Antibody (17H32L7)
ATG16L1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot 및 Immunocytochemistry에 적합합니다. 높은 특이성과 재현성을 제공하며, 단백질 A로 정제된 액상 형태입니다. 인간, 생쥐, 랫드 시료에 반응하며, 연구용으로 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1–4 µg/mL
Immunocytochemistry (ICC/IF)
- Tested Dilution: 2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 17H32L7 |
| Immunogen | Full length protein corresponding to Human ATG16L1 (aa 1–607, UniProt ID: Q676U5-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2607657 |
Product Specific Information
This antibody is predicted to react with Monkey, Rat, Mouse, and Rabbit.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
Autophagy related protein 16L (ATG16L1) is encoded by the ATG16L gene located on chromosome 2 in humans. It is also known as WD30 or IBD10 (Inflammatory Bowel Disease 10).
ATG12 and ATG5 conjugate and oligomerize with ATG16L to form the Atg16L complex (~800 kDa). This complex facilitates the coupling or lipidation of LC3 to phosphatidylethanolamine (PE), a key step in autophagy. The ATG16L complex, along with other ATGs such as ATG18 and ATG21, prevents premature cleavage of ATG8-PE by the cysteine protease Atg4 until autophagosome maturation is complete.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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