Thermo Fisher Scientific SPAG4 Monoclonal Antibody (OTI3E9), TrueMAB
인간 SPAG4 단백질(aa 191-279)을 항원으로 제작된 Mouse IgG2a 단일클론 항체. Western blot에 1:500 희석으로 검증됨. 무보존제, 동결건조 형태로 제공되며 연구용으로만 사용 가능.
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Applications
- Western Blot (WB): Tested dilution 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3E9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 191–279 of human SPAG4 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of ~1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers (midpiece and principal piece) and the fibrous sheath (principal piece of the tail).
Defects in these structures are linked to abnormal tail morphology, reduced sperm motility, and infertility.
In rats, the SPAG4 protein associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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