Thermo Fisher Scientific Biotin Polyclonal Antibody, AP
Western blot, IHC, ELISA, IP 등 다양한 응용에 적합한 비오틴 폴리클로날 항체. 알칼리 포스파타제(AP) 결합형으로 높은 감도와 특이성을 제공. 4°C에서 안정하며, 동결 금지. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| ELISA | 1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:2,500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Biotin conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Alkaline Phosphatase (AP) |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.05M Tris-HCl, pH 8, with 10 mg/mL BSA, 0.15M NaCl, 0.0001M ZnCl₂, 0.001M MgCl₂, 50% glycerol |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C before opening. Do not freeze.
- Stable at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
- Freezing alkaline phosphatase conjugates will result in substantial loss of enzymatic activity.
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, and Biotinylated IgG.
Target Information
Biotin (Vitamin B7) is a water-soluble B complex vitamin required by all organisms. It can only be synthesized by bacteria, yeasts, molds, algae, and certain plants. Biotin is essential for incorporating carbon dioxide into organic compounds and has a molecular weight of approximately 244 Da.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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