Thermo Fisher Scientific IL-8 (1-77) (CXCL8) Monoclonal Antibody (8CH), eFluor 450, eBioscience

Thermo Fisher Scientific IL-8 (1-77) (CXCL8) Monoclonal Antibody (8CH), eFluor 450, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular Staining)
• Tested Dilution: 5 µL (0.06 µg)/test
  A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL (10⁵–10⁸ cells/test).

Publications

• View 1 publication

Product Specifications

Available Formats

• Alexa Fluor™ 488
• APC
• PE
• PerCP-eFluor™ 710
• Request custom conjugation

Product Specific Information

Description:
The 8CH monoclonal antibody reacts with human IL-8 (CXCL8), a pro-inflammatory CXC chemokine. IL-8 is synthesized as a 99-amino acid precursor and processed into several isoforms, most commonly 72 or 77 amino acids. IL-8(77) is mainly secreted by endothelial cells and is involved in angiogenesis during fetal development. IL-8(72), predominant in adults, is secreted by monocytes, macrophages, epithelial cells, and fibroblasts in response to inflammatory stimuli. It plays a key role in neutrophil activation and recruitment, as well as T cell migration, signaling through CXCR1 and CXCR2 receptors. IL-8 expression is often elevated in tumors and is associated with angiogenesis and metastasis.

Applications Reported:

• Intracellular staining followed by flow cytometric analysis.

Applications Tested:

• Pre-titrated and validated for intracellular flow cytometry at 5 µL (0.06 µg)/test.

Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emitting at 445 nm.
Ensure the instrument can detect this fluorochrome.

• Excitation: 405 nm
• Emission: 445 nm
• Laser: Violet Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Interleukin 8 (IL-8, CXCL8):
A 72-amino acid pro-inflammatory chemokine belonging to the CXC subfamily. It binds to IL-8RA and IL-8RB receptors and acts as a chemoattractant and angiogenic factor. IL-8 expression is induced by inflammatory stimuli in various cells including macrophages and endothelial cells, where it is stored in Weibel-Palade bodies. IL-8 signals through CXCR1 and CXCR2 receptors and plays roles in tumor angiogenesis and inflammation-related diseases such as bronchiolitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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