Thermo Fisher Scientific SPACA3 Polyclonal Antibody, FITC
SPACA3 단백질을 표적하는 FITC 결합 폴리클로날 항체로, Western Blot, ELISA, IP에 적합합니다. 인간, 생쥐, 랫트 반응성이 있으며, 고순도 친화성 크로마토그래피로 정제되었습니다. 형광 스펙트럼은 498/517 nm이며 연구용으로만 사용됩니다.
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Thermo Fisher Scientific SPACA3 Polyclonal Antibody, FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptides within amino acid regions 50–100 and 80–130 on human sperm acrosome membrane-associated protein 3 isoform 2 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
SPACA3 (sperm acrosome associated 3), also known as sperm lysozyme-like protein 1, lysozyme-like protein 3, lysozyme-like acrosomal sperm-specific secretory protein ALLP-17, cancer/testis antigen 54 (CT54), LYC3, SPRASA or LYZL3, is a 215 amino acid protein that participates in the fusion and adhesion of sperm and egg plasma membrane during fertilization.
Identified as a novel cancer/testis antigen in hematologic malignancies, SPACA3 can elicit B-cell immune responses in cancer patients and is considered a potential target for immunotherapy.
A member of the glycosyl hydrolase 22 family, SPACA3 is expressed in testis, placenta, and epididymis. It exists as two alternatively spliced isoforms:
- Isoform 1: single-pass type II membrane protein of the sperm acrosome
- Isoform 2: secreted protein
SPACA3 may act as a receptor for egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. The processed form has no detectable bacteriolytic activity in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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