Thermo Fisher Scientific ZO-1 Polyclonal Antibody
ZO-1 단백질 검출용 Thermo Fisher Scientific의 폴리클로날 항체. WB, IHC, ICC 등 다양한 응용 가능. 인간 및 마우스 반응성, 고순도 항원 친화 크로마토그래피 정제. 장기 보관 시 -20°C에서 안정적 유지.
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Thermo Fisher Scientific ZO-1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 6 publications |
| Immunohistochemistry (IHC) | – | View 4 publications |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | – | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse, Pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human ZO-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.12 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792399 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (95%), Rat (94%), Dog (95%).
- Positive Control: 293T, A431, HeLa, HepG2, A549, H1299, HCT116, mouse testis.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ZO-1 forms complexes with either ZO-2 or ZO-3. These proteins can associate with claudin, occludin, and F-actin at tight junction strands, providing linkage between the actin cytoskeleton and tight junctions.
ZO-1 expression is reduced in many breast cancer lines.
ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, while ZO-1 is also expressed at cell junctions of cardiac myocytes.
ZO-1 is located on the cytoplasmic membrane surface of intercellular tight junctions and may be involved in signal transduction at cell-cell junctions.
Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene.
Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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