Thermo Fisher Scientific ACE Recombinant Rabbit Monoclonal Antibody (JM59-32)
ACE 단백질을 인식하는 recombinant rabbit monoclonal antibody (Clone JM59-32). Western blot, IHC, Flow cytometry에 적합하며, 높은 특이성과 재현성을 제공. Protein A 정제, 1 mg/mL 농도의 액상 형태로 제공되며, 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 | - |
| Flow Cytometry (Flow) | 1:50–1:100 | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM59-32 |
| Immunogen | Synthetic peptide within Human ACE aa 1257–1306 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810018 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed in HEK293 cells. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
The ACE gene encodes an enzyme that converts angiotensin I into angiotensin II, a potent vasopressor and aldosterone-stimulating peptide critical for blood pressure regulation and fluid-electrolyte balance.
This enzyme is a key component of the renin-angiotensin system. Variations in the gene, including a 287 bp Alu repeat element, have been linked to enzyme levels and cardiovascular pathophysiology.
Two main alternatively spliced isoforms (somatic and testicular) are equally active, with additional variants identified but not fully characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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