Thermo Fisher Scientific Complement C5a Monoclonal Antibody (2952)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunohistochemistry (Frozen) (IHC (F))

1:50

-

ELISA (ELISA)

1:50

View 1 publication 1 publication

Functional Assay (Functional)

1:50

-

Product Specifications

Species Reactivity

Human

Published species

Cynomolgus monkey

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2952

Immunogen

Neo-epitope on C5a.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.1% BSA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1073838

Product Specific Information

MA1-40162 detects C5a/C5a des-Arg from human samples.

MA1-40162 has been successfully used in ELISA, Immunohistochemsitry (Frozen, Paraffin), Functional Assays and Western blot applications.

The MA1-40162 immunogen is the Neo-epitope on C5a.

The MA1-40162 immunogen is the Neo-epitope on C5a (ISHKDMQLG amino acid 65-73).

Endotoxin <24 EU/mg.

Positive control Human Granulocytes.

General comment: Please notice that under given conditions it is known that C5 can expose epitopes normally only found in the cleaved activation products

Target Information

The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to a susceptibility to liver fibrosis and to rheumatoid arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.