Thermo Fisher Scientific H4K16ac Recombinant Rabbit Monoclonal Antibody (JB21-44)
H4K16ac에 특이적인 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. HEK293 세포에서 발현된 IgG 형 항체로 높은 특이성과 재현성을 제공. 단백질 A로 정제되었으며 1 mg/mL 농도로 액상 형태로 공급.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JB21-44 |
| Immunogen | Recombinant protein within Human Histone H4 (acetyl K16), aa 1–50 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | −20°C, avoid freeze/thaw cycles, store in dark |
| Shipping Conditions | Wet ice |
| RRID | AB_2848625 |
Product Specific Information
Positive Control: Siha, Hela, SH-SY-5Y, rat brain tissue, human tonsil tissue, human lung cancer tissue, mouse colon tissue.
Target Information
Histones are basic nuclear proteins responsible for forming the nucleosome structure of eukaryotic chromosomal fibers. Two molecules of each core histone (H2A, H2B, H3, and H4) form an octamer around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and aids chromatin compaction into higher-order structures. The H4 gene is intronless, encodes a member of the histone H4 family, and contains a palindromic termination element instead of a polyA tail. It is located within the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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