Thermo Fisher Scientific BCL9L Monoclonal Antibody (1C9G2)
BCL9L 단백질을 인식하는 Mouse monoclonal 항체로, Western blot, Flow cytometry, ELISA에 적합합니다. 정제된 human BCL9L 단백질(AA 606-751)을 면역원으로 사용하였으며, Protein G로 정제된 액상 형태입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1C9G2 |
| Immunogen | Purified recombinant fragment of human BCL9L (AA: 606–751) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787273 |
Product Specific Information
MA5-31649 has been tested in indirect ELISA.
Target Information
Bcl9L, a homolog of Bcl9, was initially identified through bioinformatics screening. It is expressed in fetal brain, adult lung, eye, and prostate, as well as in several tumor types including pancreatic and prostate cancers.
Bcl9L interacts with β-catenin, a target of the Wnt signaling pathway, and is required for enhanced β-catenin–T-cell factor (TCF)-mediated transcription in colorectal tumor cells, possibly by translocating β-catenin to the nucleus.
Studies have shown that Bcl9L expression correlates with high nuclear grade cancer phenotype and ErbB2/HER-2 expression in breast cancers, suggesting potential roles in other cancer types.
Bcl9L is also critical for Wnt-mediated regulation of stem cell traits in colon epithelium and adenocarcinomas, which are associated with tumor invasion, metastasis, and therapy resistance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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