Thermo Fisher Scientific alpha Adaptin Monoclonal Antibody (AP6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

View 10 publications 10 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 32 publications 32 publications

Flow Cytometry (Flow)

1 µg / 10^6 cells

-

Immunoprecipitation (IP)

Assay-dependent

View 3 publications 3 publications

in situ PLA (PLA)

-

View 1 publication 1 publication

Inhibition Assays (IA)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Hamster, Human, Mouse, Non-human primate, Rat

Published species

Bovine, Hamster, Human, Mouse, Non-human primate, Pig, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

AP6

Immunogen

Purified adaptors. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:alpha Adaptin,uniProtId:O95782-1,ncbiNodeId:9606,antigenRange:1-977,antigenLength:977,antigenImageFileName:MA1-064_alpha_Adaptin_O95782-1_House_mouse.svg,antigenImageFileNamePDP:MA1-064_alpha_Adaptin_O95782-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 647
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2258307

Product Specific Information

MA1-064 detects assembly polypeptide 2 (AP2) from a wide variety of mammalian sources, including human, hamster, monkey, bovine, rat, and mouse. It recognizes the products of both alpha-adaptin genes, alpha A and alpha C as well as an alternatively spliced isoform of alpha adaptin found in neurons.

MA1-064 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects an ~100 kDa doublet representing the two isoforms of alpha adaptin in rat brain (alpha C is the dominant form seen)

Microinjection of cells with MA1-064 results in variable blockade of endocytosis.

The MA1-064 immunogen is purified adaptors.

Target Information

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.