Thermo Fisher Scientific Phospho-FOXO4 (Thr455) Polyclonal Antibody
FOXO4 단백질의 Thr455 인산화 형태를 특이적으로 인식하는 rabbit polyclonal antibody. WB, IHC, ICC/IF에 사용 가능하며 인간, 마우스, 랫트에 반응. PBS/glycerol buffer에 보관되며 -20°C에서 안정적. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human FOXO4 (Accession P98177), corresponding to amino acid residues around phosphorylated Thr455 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2903141 |
Product Specific Information
This antibody detects endogenous levels of FOXO4 only when phosphorylated at Thr455.
Target Information
FOXO4 (Forkhead box O4) is encoded by the FOXO4 gene located on the X chromosome. Also known as AFX1 or MLLT7, it belongs to the O class of forkhead/winged helix family of transcription factors, along with FOXO1, FOXO3, and FOXO6.
FOXO4 is abundantly expressed in skeletal muscle and adipose tissue. It binds to the Insulin Responsive Element (IRE) and activates downstream targets of Insulin/IGF1 signaling such as IGFBP1. Activation of Akt, another downstream target of the Insulin/IGF1 pathway, leads to phosphorylation of FOXO4 on specific Ser/Thr residues, promoting its binding to 14-3-3 and subsequent nuclear export.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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