Thermo Fisher Scientific Cholesterol Oxidase Polyclonal Antibody
Cholesterol Oxidase에 특이적인 염소 유래 폴리클로날 항체로, WB, ELISA, IP 등 다양한 면역분석에 사용 가능. 고순도 정제 및 안정적 보관 가능하며, 연구용으로 적합. 박테리아 반응성 및 비결합형 형태로 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| ELISA | 1:5,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Cholesterol Oxidase [Streptomyces] |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Anti-Cholesterol Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, ion-exchange chromatography, and extensive dialysis.
Immunoelectrophoresis assay shows a single precipitin arc against anti-Goat Serum and purified Cholesterol Oxidase [Microorganism].
Cross-reactivity with Cholesterol Oxidase from other sources is unknown.
Target Information
Cholesterol Oxidase is a bifunctional enzyme that catalyzes the oxidation of the 3‑beta‑hydroxy group of cholesterol and the isomerization of the double bond of the resulting product.
This enzyme participates in cholesterol metabolism, a part of steroid metabolism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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