Thermo Fisher Scientific N-Acylmannosamine-1-Dehydrogenase Polyclonal Antibody
N-Acylmannosamine-1-Dehydrogenase를 인식하는 염소 유래 폴리클로날 항체. Western blot, ELISA, IP에 사용 가능. 정제된 IgG로 높은 특이성과 재현성 제공. 세균 단백질 연구용으로 적합하며, 장기 보관 시 -20°C 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| ELISA | 1:5,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | N-Acyl Mannosamine-1-Dehydrogenase (Recombinant sequence from Pseudomonas expressed in E. coli) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Anti-N-Acylmannosamine-1-Dehydrogenase antibody is an IgG fraction purified from monospecific antiserum via a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis.
Immunoelectrophoresis shows a single precipitin arc against anti-Goat Serum and purified N-Acylmannosamine-1-Dehydrogenase (Pseudomonas).
Cross-reactivity with other sources is unknown.
Target Information
N-acylmannosamine 1-dehydrogenase acts on acetyl-D-mannosamine and glycolyl-D-mannosamine.
NAM-DH may be applicable for quantitative determination of sialic acid in serum.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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