Thermo Fisher Scientific Galectin 3 Polyclonal Antibody, PeproTech
Human Galectin-3 인식 Rabbit Polyclonal Antibody로 Western Blot, IHC, ELISA에 사용 가능. 항원 친화 크로마토그래피로 정제된 고순도 항체. Recombinant Human Galectin-3을 검출하는 연구용 시약. -20°C 보관, PBS buffer, 무보존제.
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Thermo Fisher Scientific Galectin 3 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| Immunohistochemistry (IHC) | – | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rat |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human Galectin-3 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930032 |
Product Specific Information
AA Sequence of Recombinant Protein:
ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYHGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSAPGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Galectin-3. Anti-Human Galectin-3-specific antibody was purified by affinity chromatography employing an immobilized Human Galectin-3 matrix.
Sandwich ELISA:
To detect Human Galectin-3 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Galectin-3 (500-P246Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human Galectin-3.
Western Blot:
To detect Human Galectin-3 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human Galectin-3 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cell migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus, acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes and macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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