Thermo Fisher Scientific GITRL Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
METAKEPCMA KFGPLPSKWQ MASSEPPCVN KVSDWKLEIL QNGLYLIYGQ VAPNANYNDV APFEVRLYKN KDMIQTLTNK SKIQNVGGTY ELHVGDTIDL IFNSEHQVLK NNTYWGIILL ANPQFIS.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human AITRL. Anti-Human AITRL-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hAITRL by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human AITRL (500-P244) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hAITRL.

Western Blot:
To detect hAITRL by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hAITRL is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package:
500-P244BT-1MG will be provided as 2 × 500 µg.

Target Information

The tumor necrosis factor (TNF) and TNF receptor (TNFR) gene superfamilies regulate cell proliferation, differentiation, and survival through activation of NF-kappa-B and mitogen-activated protein kinases.
GITR (Glucocorticoid-Induced TNF Receptor) is expressed on CD4⁺ CD25⁺ regulatory T cells and plays a crucial role in immune regulation.
Its ligand, GITRL, is expressed in endothelial and antigen-presenting cells and can activate NF-kappa-B, induce apoptotic and anti-apoptotic effects, inhibit Treg suppressive activity, and co-stimulate responder T cells through GITR.
Dominant negative forms of NIK and TRAF2 expressed in transfected 293 cells substantially inhibited NF-kappa-B activation, suggesting that the GITRL–GITR pathway involves both NIK and TRAF2.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.