Thermo Fisher Scientific hCG Recombinant Rabbit Monoclonal Antibody (RM330)
Recombinant rabbit monoclonal antibody specific to hCG, recognizing both native complex and beta subunit. Optimized for WB, IHC, and ELISA. Provides high specificity, sensitivity, and lot-to-lot consistency. Suitable for research use only.
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Thermo Fisher Scientific hCG Recombinant Rabbit Monoclonal Antibody (RM330)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
| ELISA | 1:1,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM330 |
| Immunogen | Native hCG protein purified from Human Urine |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2810129 |
Product Specific Information
Specificity: Reacts to hCG (human Chorionic Gonadotropin). The antibody recognizes both the native complex and the beta subunit of hCG.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Human chorionic gonadotropin (hCG) is a placental hormone that stimulates secretion of the pregnancy-sustaining steroid progesterone. hCG belongs to a family of glycoprotein hormones including lutropin (LH), follitropin (FSH), and thyrotropin (TSH). These hormones are disulfide-rich heterodimers composed of alpha and beta subunits. The alpha subunit is common among species, while the beta subunit confers receptor and biological specificity.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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