Thermo Fisher Scientific H3K27me3 Recombinant Rabbit Monoclonal Antibody (RM175)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunohistochemistry (IHC)

0.5-2 µg/mL

-

ELISA (ELISA)

0.5-1 µg/mL

-

ChIP assay (ChIP)

1-5 µg

-

Luminex (LUM)

0.1-0.5 µg/mL

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Recombinant Monoclonal

Type

Antibody

Clone

RM175

Immunogen

A trimethyl-peptide corresponding to trimethyl-Histone H3 (Lys27).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2866508

Product Specific Information

Specificity: reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity non-modified Lysine 27, monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in histone H3.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

H3K27me3 is the epigenetic mark where histone H3 is trimethylated at lysine residue 27. H3K27me3 is an epigenetic silencing mark and thus associated with downregulation of nearby genes and often found in heterochromatin regions. H3K27me3 is mainly found at inactive gene promoters and generally is found in opposition to the activating mark H3K4me3. H3K27me3 often occupies gene rich regions with close association to development regulators. EZH2, a component of the PRC2 complex, is the histone methyltransferase (or writer) responsible for trimethylating H3K27. UTX and JMJD3 both are histone demethylases (or erasers) for H3K27me3. The regulation of H3K27me3 is crucial for gene regulation and dysregulation of H3K27me3 has been implicated in numerous diseases including cancer. H3K27me3 antibodies are often used for western blot and ChIP applications to study changes in levels and occupancy of H3K27me3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.