Thermo Fisher Scientific Nuclear Antigen Monoclonal Antibody (235-1)
인간 세포에 특이적으로 반응하는 단클론 항체로, IHC 및 ICC/IF에 사용 가능. 인간 세포 핵을 염색하며 마우스나 랫 세포에는 반응하지 않음. 액상 형태로 제공되며 -20°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 235-1 |
| Immunogen | Human cell homogenate |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Buffer | Tissue culture supernatant |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2802648 |
Product Specific Information
This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells.
This clone stains nuclei of all human cell types, giving a diffuse nuclear staining pattern; chromosomes are negatively stained in metaphase cells.
With immunoprecipitation, the antibody reacts with ~80 kDa and ~70 kDa bands.
The antibody works on acetone-fixed and paraformaldehyde-fixed cells. It is suggested to permeabilize the cells with 0.1–0.4% Triton X-100.
The antibody also works for immunohistochemistry on paraformaldehyde-fixed cryostat sections.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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