Thermo Fisher Scientific Nuclear Antigen Monoclonal Antibody (235-1)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

1:100

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

235-1

Immunogen

Human cell homogenate.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage buffer

tissue culture supernatant

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2802648

Product Specific Information

This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells. This clone stains nuclei of all human cell types giving a diffuse nuclear staining pattern; chromosomes are negatively stained in metaphase cells. With immunoprecipitation, the antibody reacts with a ~80 kDa and ~70 kDa band. The antibody works on acetone-fixed and paraformaldehyde-fixed cells. It is suggested to permeabilize the cells with 0.1-0.4% Triton X-100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.