Thermo Fisher Scientific VSV-G Epitope Tag Polyclonal Antibody, DyLight 549
VSV-G epito프 태그를 인식하는 DyLight 549 결합 폴리클로날 항체로, Western blot, ICC/IF, ELISA에 적합. 높은 특이성과 감도를 제공하며, 친화 정제된 토끼 IgG 항체. 연구용 단백질 발현 검출에 최적화.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 501–511 (Y-T-D-I-E-M-N-R-L-G-K) of vesicular stomatitis virus glycoprotein (VSV-G) conjugated to KLH using maleimide. |
| Conjugate | DyLight™ 549 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against the VSV-G epitope tag and is useful in determining its presence in overexpressed proteins in various assays.
The antibody recognizes the VSV-G epitope tag (Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Target Information
Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding.
The glycoprotein (VSV G) contains a domain in its extracellular membrane-proximal stem that appears to be needed for efficient VSV budding.
Sequence: YTDIEMNRLGK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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