Thermo Fisher Scientific DYKDDDDK Polyclonal Antibody, DyLight 549
DYKDDDDK(FLAG) 태그 단백질 검출용 토끼 폴리클로날 항체로, DyLight 549 형광 표지. Western blot, ICC/IF, ELISA에 적합하며 높은 특이성과 감도를 제공. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | 1:10,000–1:25,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified from whole rabbit serum immunized with the Enterokinase Cleavage Site (ECS) peptide (D-Y-K-D-D-D-D-K) conjugated to KLH. Reacts with FLAG™ conjugated proteins. |
| Conjugate | DyLight™ 549 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below. Avoid freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This affinity purified antibody is directed against the FLAG epitope tag and is used to detect its presence in overexpressed proteins in various assays. It recognizes the FLAG tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the N- or C-terminus of target proteins in transfected or transformed cells.
Target Information
The DYKDDDDK tag (FLAG tag) is a short peptide sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) widely used as a protein tag to facilitate protein purification, detection, and localization.
It can be fused to the N- or C-terminus of a protein, enabling easy identification and isolation using anti-FLAG antibodies.
The FLAG tag is hydrophilic, minimizing interference with protein folding or activity, and is used extensively in molecular biology and biochemistry research for:
- Protein-protein interaction studies
- Protein expression analysis
- Protein localization studies
FLAG™ is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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