Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Super Bright 780, eBioscience
Mouse CD11b 단백질을 인식하는 M1/70 클론의 Rat IgG2b 모노클로날 항체로, Super Bright 780 형광체로 결합되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 405 nm 레이저로 여기되어 780 nm에서 방출됩니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Super Bright 780, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 |
| Immunohistochemistry (IHC) | - | 13 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 4 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 2 |
| Immunocytochemistry (ICC/IF) | - | 6 |
| Flow Cytometry (Flow) | 0.125 µg/test | 122 |
| Neutralization (Neu) | - | 2 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host/Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 780 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M1/70 |
| Conjugate | Super Bright™ 780 |
| Excitation/Emission Max | 413/780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2722925 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV series (395–805)
- Brilliant Violet series (421–786)
- eFluor 450, 506, 660
- FITC
- Functional Grade
- NovaFluor Blue and Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702
- Custom conjugation available
Product Specific Information
The M1/70 monoclonal antibody reacts with mouse CD11b, a 165–170 kDa integrin alphaM that associates with CD18 to form the alphaMbeta2 integrin (Mac-1). It binds to CD54 (ICAM-1), C3bi, and fibrinogen. CD11b is expressed by macrophages, NK cells, granulocytes, activated lymphocytes, and mouse B-1 cells. M1/70 cross-reacts with human CD11b, allowing detection on human monocytes, granulocytes, and NK cells.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Validated on mouse bone marrow cells at ≤0.125 µg/test (100 µL final volume). Optimal antibody concentration should be titrated experimentally.
Super Bright 780 is a tandem dye excited by the 405 nm violet laser and emits at 780 nm. Use a 780/60 bandpass filter. Verify instrument compatibility.
When using multiple Super Bright conjugates, apply Super Bright Complete Staining Buffer (Product # SB-4401) to reduce non-specific interactions.
For compensation setup, use stained cells rather than beads if higher compensation values are observed.
Light Sensitivity: Protect vial and samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer or 1-step Fix/Lyse Solution with minimal impact on brightness.
Excitation: 405 nm
Emission: 780 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD11b (integrin alpha-M, ITGAM) forms a heterodimer with CD18 known as Mac-1 or CR3. It is expressed on monocytes/macrophages, granulocytes, lymphocytes, NK cells, dendritic cells, and microglia. CD11b/CD18 mediates adhesion via ICAMs, CD14, CD50, CD23, fibrinogen, and Factor X. It serves as a microglial marker and varies by tissue macrophage type. Dysfunctions are linked to systemic lupus erythematosus and ITGAM-related susceptibility.
For Research Use Only. Not for use in diagnostic procedures.
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