Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Super Bright 780, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Super Bright 780, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–786)
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue and Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702
• Custom conjugation available

Product Specific Information

The M1/70 monoclonal antibody reacts with mouse CD11b, a 165–170 kDa integrin alphaM that associates with CD18 to form the alphaMbeta2 integrin (Mac-1). It binds to CD54 (ICAM-1), C3bi, and fibrinogen. CD11b is expressed by macrophages, NK cells, granulocytes, activated lymphocytes, and mouse B-1 cells. M1/70 cross-reacts with human CD11b, allowing detection on human monocytes, granulocytes, and NK cells.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Validated on mouse bone marrow cells at ≤0.125 µg/test (100 µL final volume). Optimal antibody concentration should be titrated experimentally.

Super Bright 780 is a tandem dye excited by the 405 nm violet laser and emits at 780 nm. Use a 780/60 bandpass filter. Verify instrument compatibility.

When using multiple Super Bright conjugates, apply Super Bright Complete Staining Buffer (Product # SB-4401) to reduce non-specific interactions.
For compensation setup, use stained cells rather than beads if higher compensation values are observed.

Light Sensitivity: Protect vial and samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer or 1-step Fix/Lyse Solution with minimal impact on brightness.

Excitation: 405 nm
Emission: 780 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD11b (integrin alpha-M, ITGAM) forms a heterodimer with CD18 known as Mac-1 or CR3. It is expressed on monocytes/macrophages, granulocytes, lymphocytes, NK cells, dendritic cells, and microglia. CD11b/CD18 mediates adhesion via ICAMs, CD14, CD50, CD23, fibrinogen, and Factor X. It serves as a microglial marker and varies by tissue macrophage type. Dysfunctions are linked to systemic lupus erythematosus and ITGAM-related susceptibility.

For Research Use Only. Not for use in diagnostic procedures.

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