Thermo Fisher Scientific HLA-G (soluble) Monoclonal Antibody (2A12), FITC
인간 HLA-G5 및 HLA-G6 분비형을 구분하는 FITC 결합 단일클론 항체입니다. Flow Cytometry에 적합하며 교차 반응 없이 특이적 검출이 가능합니다. 액상 형태로 제공되며 4°C에서 암소 보관합니다.
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Thermo Fisher Scientific HLA-G (soluble) Monoclonal Antibody (2A12), FITC
Applications
- Flow Cytometry (Flow)
Tested Dilution: 1–5 µg/mL
Publications: (참조 없음)
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2A12 |
| Immunogen | C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153013 |
Additional Formats
Product Specific Information
This antibody was generated to a peptide corresponding to C-intron 4-encoded sequence.
It does not cross-react with the full-length HLA-G1 isoform and thus allows distinction between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.
Target Information
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane and is approximately 45 kDa.
The gene contains 8 exons:
- Exon 1: leader peptide
- Exons 2–3: alpha1 and alpha2 domains (peptide-binding)
- Exon 4: alpha3 domain
- Exon 5: transmembrane region
- Exon 6: cytoplasmic tail
HLA-G is expressed on fetal-derived placental cells.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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