Thermo Fisher Scientific HLA-G Monoclonal Antibody (87G)
HLA-G 단백질을 인식하는 mouse monoclonal antibody (clone 87G). 인간 시료에 반응하며, WB, IHC, Flow, ELISA에 사용 가능. 비결합형(unconjugated)으로 제공되며, PBS(pH 7.4) 용액 내 1 mg/mL 농도. 연구용 한정.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC-F) | 10 µg/mL | - |
| Flow Cytometry (Flow) | 2 µg/mL | - |
| ELISA | 1 µg/mL | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Pig |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 87G |
| Immunogen | HLA-B27 transgenic mice immunized with H-2 identical murine cells transfected with genes encoding HLA-G and human β2-microglobulin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153222 |
Available Conjugate Formats
Product Specific Information
This antibody recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5).
HLA-G is a nonclassical MHC Class I molecule expressed on trophoblast cells.
It does not cross-react with mouse or rat.
Endotoxin level is less than 0.01 EU/µg of protein (LAL test).
For IHC (Frozen): incubation 20 min at 25°C; functional application includes blocking.
Target Information
HLA-G belongs to the HLA Class I heavy chain family. It forms a heterodimer with β2-microglobulin and is expressed on fetal-derived placental cells.
The heavy chain (~45 kDa) gene has 8 exons:
- Exon 1: leader peptide
- Exons 2–3: α1, α2 domains (peptide-binding)
- Exon 4: α3 domain
- Exon 5: transmembrane region
- Exon 6: cytoplasmic tail
HLA and MHC antibodies are important in Immunopeptidomics for identifying and characterizing neoantigens via HPLC-MS/MS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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