Thermo Fisher Scientific HLA-G (soluble) Monoclonal Antibody (5A6G7), Biotin
인간 HLA-G5 및 HLA-G6 분비형 단백질을 특이적으로 인식하는 단클론 항체로, Biotin으로 표지되어 다양한 면역분석에 활용 가능. Western blot, IHC, Flow cytometry 등 다중 응용에 적합하며, HLA-G1과 교차반응하지 않아 isoform 구분에 유용. 연구용으로만 사용.
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Thermo Fisher Scientific HLA-G (soluble) Monoclonal Antibody (5A6G7), Biotin
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 1–4 µg/mL |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5A6G7 |
| Immunogen | C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11151937 |
Product Specific Information
This antibody was generated to a peptide corresponding to the C-intron 4-encoded sequence. It does not cross-react with the full-length HLA-G1 isoform and thus allows distinction between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.
Target Information
HLA-G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain (~45 kDa) is membrane-anchored and encoded by a gene containing 8 exons:
- Exon 1: Leader peptide
- Exons 2–3: α1 and α2 domains (peptide binding)
- Exon 4: α3 domain
- Exon 5: Transmembrane region
- Exon 6: Cytoplasmic tail
HLA-G is expressed on fetal-derived placental cells.
HLA and MHC antibodies play a significant role in immunopeptidomics, enabling identification and characterization of neoantigens via high-performance liquid chromatography coupled to tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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