Thermo Fisher Scientific CD16 Monoclonal Antibody (DJ130c)

Applications

Product Specifications

Product Specific Information

This antibody recognizes an epitope in the first membrane-distal domain of CD16 and detects both CD16A and CD16B.
Heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) is recommended for paraffin sections.
A suggested positive control is human tonsil.
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10^6 cells in 100 µL.

Target Information

CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in clearance of immune complexes and cellular mediated cytotoxicity.
The gene FCGR3A shares high similarity with FCGR3B, both located on chromosome 1.

• FCGR3A: expressed on NK cells, anchored via transmembrane peptide
• FCGR3B: expressed on PMN cells, anchored via phosphatidylinositol (PI) linkage

Mutations in FCGR3A are associated with:

• Immunodeficiency 20
• Susceptibility to recurrent viral infections
• Systemic lupus erythematosus
• Alloimmune neonatal neutropenia

Alternatively spliced variants encoding different isoforms have been identified.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.