Thermo Fisher Scientific CD16 Monoclonal Antibody (DJ130c)
인간 CD16A 및 CD16B를 인식하는 마우스 모노클론 항체로, IHC, ICC/IF, Flow Cytometry에 사용 가능. 단백질 A로 정제된 액상 형태이며 1 mg/mL 농도. 면역복합체 제거 및 세포매개 세포독성 연구에 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 1:50–1:200 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DJ130c |
| Immunogen | CD16 antigen |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2104009 |
Product Specific Information
This antibody recognizes an epitope in the first membrane-distal domain of CD16 and detects both CD16A and CD16B.
Heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) is recommended for paraffin sections.
A suggested positive control is human tonsil.
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10^6 cells in 100 µL.
Target Information
CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in clearance of immune complexes and cellular mediated cytotoxicity.
The gene FCGR3A shares high similarity with FCGR3B, both located on chromosome 1.
- FCGR3A: expressed on NK cells, anchored via transmembrane peptide
- FCGR3B: expressed on PMN cells, anchored via phosphatidylinositol (PI) linkage
Mutations in FCGR3A are associated with:
- Immunodeficiency 20
- Susceptibility to recurrent viral infections
- Systemic lupus erythematosus
- Alloimmune neonatal neutropenia
Alternatively spliced variants encoding different isoforms have been identified.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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