Thermo Fisher Scientific CD8 alpha Monoclonal Antibody (53-6.7)

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Flow Cytometry (Flow)

1:20

View 30 publications 30 publications

Immunoprecipitation (IP)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 8 publications 8 publications

Product Specifications

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

53-6.7

Immunogen

Murine thymus or spleen. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD8,uniProtId:P01731-1,ncbiNodeId:10090,antigenRange:1-247,antigenLength:247,antigenImageFileName:MA1-84018_CD8_P01731-1_Rat.svg,antigenImageFileNamePDP:MA1-84018_CD8_P01731-1_Rat_PDP.jpeg,sortOrder:2},{targetFamily:CD8,uniProtId:P10300-1,ncbiNodeId:10090,antigenRange:1-213,antigenLength:213,antigenImageFileName:MA1-84018_CD8_P10300-1_Rat.svg,antigenImageFileNamePDP:MA1-84018_CD8_P10300-1_Rat_PDP.jpeg,sortOrder:5}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

4°C or -20°C if preferred

Shipping conditions

Wet ice

RRID

AB_2075138

Product Specific Information

MA1-84018 is recommended for IHC using acetone-fixed frozen tissue sections. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL. This antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells.

Rat anti Mouse CD8 alpha antibody, clone 53-6.7 detects mouse CD8a, also known as Lyt-2.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.