Thermo Fisher Scientific RANKL (soluble) Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein
MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID.

Preparation
Produced from sera of rabbits immunized with highly pure Recombinant Human sRANK Ligand. Anti-Human sRANK Ligand-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA
To detect Human sRANK Ligand by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sRANK Ligand (500-P133) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human sRANK Ligand.

Western Blot
To detect hsRANKL by Western blot, use at 0.1–0.2 µg/mL.
With compatible secondary reagents, detection limit for Recombinant hsRANKL is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Packaging
500-P133BT-1MG is provided as 2 × 500 µg.

Target Information

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family, a ligand for osteoprotegerin and key factor for osteoclast differentiation and activation. It functions as a dendritic cell survival factor and regulates T cell–dependent immune responses.
T cell activation induces expression of this gene, increasing osteoclastogenesis and bone loss. The protein activates antiapoptotic kinase AKT/PKB through a complex involving SRC kinase and TRAF6, suggesting a role in apoptosis regulation.
Gene disruption in mice causes severe osteopetrosis, lack of osteoclasts, defects in early T/B lymphocyte differentiation, and failure to form mammary structures during pregnancy. Two alternatively spliced transcript variants have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.