Thermo Fisher Scientific LMW E.Coli Host Cell Proteins Polyclonal Antibody
E.coli 유래 저분자량 숙주세포단백질(LMW HCP)을 검출하기 위한 Rabbit Polyclonal Antibody로, WB, ELISA, IP에 사용 가능. 비결합형 액상 형태이며, -20°C 보관. 연구용으로 생물학적 제제의 불순물 분석에 적합.
- 카탈로그번호
- 100-401-J06
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific LMW E.Coli Host Cell Proteins Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:1,000–1:2,000 |
| Immunoprecipitation (IP) | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-LMW E. coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture of low molecular weight (LMW) proteins from E. coli (BL21). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 72.5 mg/mL |
| Storage Buffer | 0.02M potassium phosphate / whole serum, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Reactivity is directed against low molecular weight E. coli Host Cell Proteins.
Cross-reactivity with other species is likely but has not been determined.
Reactivity against homologues from other sources is not known.
Target Information
Host Cell Proteins (HCPs) are process-related impurities derived from host cell expression systems that may be present in trace amounts in final drug substances.
HCP Antibodies are used to detect impurities in biologically based therapeutics.
2D electrophoresis western blot analysis provides more complete insight into the immunocoverage of protein components of the host cell lysate, beyond what can be detected by 1D electrophoresis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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