Thermo Fisher Scientific Phospho-PRKAR2A (Ser96) Polyclonal Antibody
인간 PRKAR2A 단백질의 Ser96 인산화 부위를 인식하는 Rabbit polyclonal 항체. Western blot에 1:500~1:3,000 희석으로 사용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공. 연구용으로 세포 신호전달 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:3,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human PKA R2 (phospho Ser96). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736407 |
Product Specific Information
- Positive Control: 293T
- Predicted Reactivity: Chimpanzee (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
cAMP is a signaling molecule important for a variety of cellular functions. It exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins.
The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes dissociation of the holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.
Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit and may interact with various A-kinase anchoring proteins to determine the subcellular localization of AMPK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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