Thermo Fisher Scientific Phospho-PKC alpha (Thr497) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000

Product Specifications

Product Specific Information

• Positive Control: Jurkat, Jurkat (CIP)
• Predicted Reactivity: Mouse (100%), Rat (100%), Rabbit (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity.
PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol.
PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. They also serve as major receptors for phorbol esters, a class of tumor promoters.
Each member has a specific expression profile and distinct cellular role. The encoded kinase plays roles in cell adhesion, transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest a role in cardiac contractility and Ca²⁺ handling in myocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.