Thermo Fisher Scientific Phospho-PKC alpha (Thr497) Polyclonal Antibody
PKC alpha (Thr497) 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, Western blot에 최적화됨. Human에 반응하며 Mouse, Rat 등과 예측 교차반응. 항원 친화 크로마토그래피로 정제되었으며, PBS/BSA/glycerol buffer에 보존.
- 판매단위
- pk
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:3,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PKC alpha (phospho Thr497). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.44 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736409 |
Product Specific Information
- Positive Control: Jurkat, Jurkat (CIP)
- Predicted Reactivity: Mouse (100%), Rat (100%), Rabbit (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity.
PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol.
PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. They also serve as major receptors for phorbol esters, a class of tumor promoters.
Each member has a specific expression profile and distinct cellular role. The encoded kinase plays roles in cell adhesion, transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest a role in cardiac contractility and Ca²⁺ handling in myocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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