Thermo Fisher Scientific PlGF-1 Polyclonal Antibody, PeproTech
Rabbit polyclonal antibody specific for Human PlGF-1, suitable for Western blot, ELISA, and blocking assays. Affinity purified for high specificity. Lyophilized form, stable at -20°C. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.5–2.0 µg/mL | 1 publication |
| Blocking Assay (BLOCK) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human PlGF-1 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929906 |
Product Specific Information
AA Sequence of recombinant protein:
MLPAVPPQQW ALSAGNGSSE VEVVPFQEVW GRSYCRALER LVDVVSEYPS EVEHMFSPSC VSLLRCTGCC GDENLHCVPV ETANVTMQLL KIRSGDRPSY VELTFSQHVR CECRPLREKM KPERCGDAVP RR
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human PlGF-1. Anti-Human PlGF-1-specific antibody was purified by affinity chromatography employing an immobilized Human PlGF-1 matrix.
Sandwich ELISA:
To detect Human PlGF-1 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human PlGF-1 (500-P226Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human PlGF-1.
Western Blot:
To detect Human PlGF-1 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Human PlGF-1 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
PlGF-1 is an angiogenic factor that belongs to the cysteine-knot superfamily of growth factors. It is expressed in placental tissues, the colon, and mammary carcinomas. PlGF-1 signals through the VEGFR-1/FLT1 receptor and stimulates endothelial cell proliferation and migration.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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