Thermo Fisher Scientific GRP94 Monoclonal Antibody (6B12), APC
GRP94 단백질을 인식하는 APC 결합 단클론 항체로, Western blot에 최적화되어 있습니다. 1:1,000 희석으로 HeLa 세포에서 GRP94 검출에 사용됩니다. 651/660 nm 형광 특성을 가지며, 4°C에서 보관합니다. 연구용으로만 사용 가능합니다.
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Applications
- Western Blot (WB): Tested dilution 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6B12 |
| Immunogen | KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA) |
| Conjugate | APC (Allophycocyanin) |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
A 1:1,000 dilution of MA5-45877 was sufficient for detection of GRP94 in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects a 94 kDa band corresponding to GRP94.
Target Information
GRP94 is an 803 amino acid protein belonging to the heat shock protein 90 family. It acts as a molecular chaperone involved in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulate CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in both innate and adaptive immunity, dendritic cell maturation and chemotaxis, antibody production, and cross-priming. GRP94 serves as a potential marker in breast cancer and functions as a peptide acceptor in the endoplasmic reticulum, assisting peptide loading of MHC class I molecules. Expression of GRP94 suppresses A23187-induced apoptosis and stabilizes calcium homeostasis. GRP94 is expressed in melanoma, liver metastases of colon carcinoma, and human gastric carcinoma BGC-823 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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