Thermo Fisher Scientific GRP94 Monoclonal Antibody (6B12), APC

Applications

• Western Blot (WB): Tested dilution 1:1,000

Product Specifications

Product Specific Information

A 1:1,000 dilution of MA5-45877 was sufficient for detection of GRP94 in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects a 94 kDa band corresponding to GRP94.

Target Information

GRP94 is an 803 amino acid protein belonging to the heat shock protein 90 family. It acts as a molecular chaperone involved in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulate CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in both innate and adaptive immunity, dendritic cell maturation and chemotaxis, antibody production, and cross-priming. GRP94 serves as a potential marker in breast cancer and functions as a peptide acceptor in the endoplasmic reticulum, assisting peptide loading of MHC class I molecules. Expression of GRP94 suppresses A23187-induced apoptosis and stabilizes calcium homeostasis. GRP94 is expressed in melanoma, liver metastases of colon carcinoma, and human gastric carcinoma BGC-823 cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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