Thermo Fisher Scientific CD11b/MAC-1 Monoclonal Antibody (ITGAM, 3340)
CD11b/MAC-1 단일클론 항체는 인간 ITGAM 단백질을 인식하며, WB 및 IHC 등 다양한 응용에 적합합니다. 단백질 A/G로 정제된 액상 형태로 제공되며, 보존제가 없고 -20°C 이하에서 보관합니다. 면역세포 표지 및 염증 연구에 유용합니다.
- 카탈로그번호
- 3684-MSM10-P1ABX
- 판매단위
- pk
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1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD11b/MAC-1 Monoclonal Antibody (ITGAM, 3340)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ITGAM, 3340 |
| Immunogen | Recombinant fragment (around aa941–1074) of human ITGAM protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex, known as integrin alpha-M beta-2, Mac-1, or CR3 (complement receptor 3), is expressed on:
- Monocytes/macrophages
- Granulocytes
- Activated lymphocytes
- Subsets of NK cells and dendritic cells
- Microglia in the brain
CD11b/CD18 functions as a receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X, facilitating critical cell-cell and cell-matrix interactions.
Expression details:
- 8% of spleen cells
- 44% of bone marrow cells
- <1% of thymocytes
- Commonly used as a microglial marker in nervous tissue
CD11b expression increases during monocyte maturation and varies among tissue macrophages. Peritoneal macrophages express higher CD11b levels than splenic macrophages.
Diseases associated with CD11b dysfunction include systemic lupus erythematosus and ITGAM-related susceptibility to lupus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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