Thermo Fisher Scientific Armenian Hamster IgG Isotype Control (eBio299Arm), Brilliant Ultra Violet 805, eBioscience

Thermo Fisher Scientific Armenian Hamster IgG Isotype Control (eBio299Arm), Brilliant Ultra Violet 805

Applications and Tested Dilution

• Flow Cytometry (Flow): Assay-Dependent
• Control (Ctrl): Assay-Dependent

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 615, 660
• FITC
• Functional Grade
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

• Description: eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin.
• Applications Reported: Suitable for flow cytometric analysis.
• Applications Tested: Tested on mouse splenocytes and normal human peripheral blood cells; use at same concentration as experimental antibody.
• Fluorochrome: Brilliant Ultra Violet™ 805 (BUV805) emits at 797 nm and is intended for use with ultraviolet (355 nm) laser-equipped cytometers.
• Compatibility: Use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize non-specific polymer interactions.
• Light Sensitivity: Protect vial and stained samples from light due to photo-induced oxidation.
• Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.
• Methanol Compatibility: Short-term methanol fixation is acceptable; avoid long-term methanol exposure (>1 hour).
• Excitation/Emission/Laser: 355 nm excitation, 797 nm emission, UV laser.
• Trademark Notice: BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Isotype control antibodies serve as negative controls to differentiate non-specific background signal from specific antibody signal.
They are typically used in flow cytometry but also applicable in ChIP, immunohistochemistry, and gel shift assays.
Isotype controls should match the species and isotype of the primary antibody, and if directly labeled, use the same fluorochrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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