Thermo Fisher Scientific CD11b/MAC-1 Monoclonal Antibody (ITGAM, 3340)

Thermo Fisher Scientific CD11b/MAC-1 Monoclonal Antibody (ITGAM, 3340)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3).

CD11b is expressed on:

• Monocytes/macrophages
• Granulocytes
• Activated lymphocytes
• Subsets of NK cells and dendritic cells
• Microglia in the brain

CD11b/CD18 functions as the receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X, which are critical for cell-cell and cell-matrix interactions.

Expression levels:

• 8% of spleen cells
• 44% of bone marrow cells
• <1% of thymocytes

Commonly used as a microglial marker in nervous tissue. Expression increases during monocyte maturation and varies among tissue macrophages. Peritoneal macrophages express higher levels of CD11b than splenic macrophages.

Diseases associated with CD11b dysfunction include systemic lupus erythematosus and ITGAM-related susceptibility to systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.