Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), Brilliant Ultra Violet 737, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 661
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 786
• eFluor 450
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The 2B8 monoclonal antibody reacts with mouse CD117 (c-Kit receptor, Steel factor receptor, stem cell factor receptor). This 145-kDa tyrosine kinase receptor is expressed by hematopoietic progenitor cells, mast cells, and plays a role in signaling and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse bone marrow cells at ≤0.25 µg/test
• Recommended Use: Optimize antibody titration for best performance

Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is intended for cytometers equipped with an ultraviolet (355 nm) laser. Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409) to minimize non-specific interactions.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Compatible with IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C.
Methanol Compatibility: Suitable for short-term methanol fixation (<1 hour).
Excitation: 355 nm; Emission: 732 nm; Laser: Ultraviolet Laser.

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

KIT (c-KIT) is a proto-oncogene encoding a type 3 transmembrane receptor for MGF (mast cell growth factor or stem cell factor). KIT regulates growth and activation of various hematopoietic and non-hematopoietic cells. Mutations are associated with gastrointestinal stromal tumors, mast cell disease, leukemia, and piebaldism.

Upon SCF binding, c-Kit dimerizes and autophosphorylates tyrosine residues, activating downstream signaling pathways (Ras/ERK, PI3K, PLC-γ, JAK/STAT). c-Kit activation is essential for hematopoiesis, stem cell maintenance, and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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