Thermo Fisher Scientific SMC6 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:40-1:200

-

Immunoprecipitation (IP)

20 µL/mg of lysate

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A region between residues 1050 and the C-terminus (residue 1091) of human Structural Maintenance of Chromosomes 6. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SMC6,uniProtId:Q96SB8-1,ncbiNodeId:9606,antigenRange:1050-1091,antigenLength:1091,antigenImageFileName:A300-237A_SMC6_Q96SB8-1_Rabbit.svg,antigenImageFileNamePDP:A300-237A_SMC6_Q96SB8-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

phosphate/tris citrate, pH 7.0 to 8.0

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Immunohistochemistry: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. SMC5-SMC6 complex may prevent transcription of episomal DNA, such as circular viral DNA genome (PubMed:26983541). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.