Thermo Fisher Scientific Phospho-S6 (Ser235, Ser236) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is breast carcinoma tissue.

Target Information

Ribosomes are organelles that catalyze protein synthesis and consist of a small 40S subunit and a large 60S subunit, composed of 4 RNA species and approximately 80 distinct proteins.
This gene encodes a cytoplasmic ribosomal protein that is part of the 40S subunit and belongs to the S6E family. It is a major substrate of protein kinases in the ribosome, with five C-terminal serine residues phosphorylated by different kinases.
Phosphorylation is induced by various stimuli including growth factors, tumor-promoting agents, and mitogens; dephosphorylation occurs during growth arrest.
The protein may regulate cell growth and proliferation through selective mRNA translation.
Multiple processed pseudogenes of this gene are dispersed throughout the genome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.