Thermo Fisher Scientific H3K4me1T6ph Polyclonal Antibody
H3K4me1T6ph 변형 히스톤 H3.2를 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, ChIP, Dot blot에 사용 가능. 다양한 종에 반응하며 고순도 Affinity chromatography로 정제됨. 액상 형태로 -20°C에서 보관.
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Thermo Fisher Scientific H3K4me1T6ph Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ChIP assay (ChIP) | 2–5 µg/1×10⁶ cells |
| Dot blot (DB) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 [Monomethyl Lys4, p Thr6] affinity purified antibody prepared from whole rabbit serum immunized with synthetic monomethylated/phosphorylated peptides surrounding Lysine 4 and Threonine 6 of human Histone H3.2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.55 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 30% glycerol |
| Contains | 0.01% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid repeated freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antibody reacts with human Histone H3.2. BLAST analysis suggests cross-reactivity with Human, Mouse, and C. elegans. Predicted to react with Rat, Chicken, Xenopus, Drosophila, and Plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Approximately 146 bp of DNA wraps around a nucleosome composed of pairs of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is compacted through interaction of linker histone H1 with DNA between nucleosomes to form higher-order chromatin structures.
This gene, part of a histone cluster on chromosome 1, encodes a member of the histone H3 family. Transcripts lack polyA tails and contain a palindromic termination element. The gene is duplicated within the cluster, and this record represents the telomeric copy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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